apc labeled mouse anti human cd 163  (Jackson Immuno)

Journal: Cell Cycle

Article Title: Kaposi's sarcoma-associated herpesvirus infection promotes differentiation and polarization of monocytes into tumor-associated macrophages

doi: 10.1080/15384101.2017.1356509

Figure Lengend Snippet: Incubation of monocytes with supernatant from KSHV-infected HUVECs results in cells expressing TAMs-specific markers. A, relative levels of mRNAs encoding LGMN, CD-163, CD-206, and CD-11b in un-treated monocytes (Mono) and macrophages (ϕ) resulting from incubation with supernatant from mock or KSHV-infected HUVECs. Differences in mRNA levels between ϕ-mock (Mock) and ϕ-KSHV (KSHV) with a P value < 0.05 are marked with a star. B, Western blot detection of LGMN, CD-163, and α-actin in cells described in A. C, representative histograms of cells described in (A) that were stained with fluorescently labeled anti-CD-163 antibody or control isotype IgG and subsequently analyzed by flow cytometry as described in Materials and Methods. D, mean values from 2 independent experiments of the fluorescence intensities in cells described in (A)that were stained with fluorescently labeled anti-LGMN or CD-163 and analyzed by flow cytometry as shown in C.

Article Snippet: Similar dual staining was also done with a APC-labeled mouse anti-human CD-163 (Jackson ImmunoResearch Laboratories) and PE-labeled mouse anti-human CD-16, and the corresponding isotype control IgGs.

Techniques: Incubation, Infection, Expressing, Western Blot, Staining, Labeling, Flow Cytometry, Fluorescence

Journal: Cell Cycle

Article Title: Kaposi's sarcoma-associated herpesvirus infection promotes differentiation and polarization of monocytes into tumor-associated macrophages

doi: 10.1080/15384101.2017.1356509

Figure Lengend Snippet: KSHV-induced IL-6, IL-10, and IL-13 are responsible for inducing differentiation and polarization of monocytes into TAMs. A, representative flow cytometry histograms of ϕ-mock (Mock) and ϕ-KSHV (KSHV) generated by incubation of monocytes with supernatant from mock or KSHV-infected HUVECs in the presence of control IgG or neutralizing antibodies specific for human IL-6, IL-10, and IL-13, alone or in combination. The cells were stained with fluorescently labeled anti-CD-163 antibody or control isotype IgG and subsequently analyzed by flow cytometry. Similar experiment was also done on these cells with fluorescently labeled anti-LGMN antibody or control IgG. B, mean values from 2 independent experiments of the fluorescence intensities in cells that were stained with fluorescently labeled anti-LGMN or CD-163 and analyzed as described in A.

Article Snippet: Similar dual staining was also done with a APC-labeled mouse anti-human CD-163 (Jackson ImmunoResearch Laboratories) and PE-labeled mouse anti-human CD-16, and the corresponding isotype control IgGs.

Techniques: Flow Cytometry, Generated, Incubation, Infection, Staining, Labeling, Fluorescence

Journal: Cell Cycle

Article Title: Kaposi's sarcoma-associated herpesvirus infection promotes differentiation and polarization of monocytes into tumor-associated macrophages

doi: 10.1080/15384101.2017.1356509

Figure Lengend Snippet: Validation of RNA-seq data by qRT-PCR. A, mRNA levels of IFIT1, IFI27, and RSAD2, which are 3 of the 10 most upregulated transcripts in ϕ-KSHV, in the same RNA samples from un-treated monocytes, ϕ-mock, and ϕ-KSHV that were used for RNA-seq analysis. B, mRNA levels of GAGE1, GAGE12J, and GAGE8, 3 of the 10 most downregulated transcripts ϕ-KSHV, in the RNA samples described in A. C, mRNA levels of TAMs-specific markers LGMN, CD-163, and CD-206 in the RNA samples described in A. D, mRNA levels of MMP-9 and IL-10 in the RNA samples described in A. Differences between ϕ-mock and ϕ-KSHV with a P value < 0.05 are marked with a star.

Article Snippet: Similar dual staining was also done with a APC-labeled mouse anti-human CD-163 (Jackson ImmunoResearch Laboratories) and PE-labeled mouse anti-human CD-16, and the corresponding isotype control IgGs.

Techniques: RNA Sequencing Assay, Quantitative RT-PCR